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nimble cloning

Nimble Cloning was applied for a plant expression system. (A)...
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In this study, we have developed a novel method for standardized molecular cloning. The cloning technique known as “Nimble Cloning” uses the restriction enzyme, ...
Nimble Cloning: A Simple, Versatile, and Efficient ... - Meta
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The cloning technique known as "Nimble Cloning" uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the vector and generate ...
Nimble Cloning: A Simple, Versatile, and Efficient ... - PubMed
https://pubmed.ncbi.nlm.nih.gov › ...
The cloning technique known as "Nimble Cloning" uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the ...
Nimble Cloning: A Simple, Versatile, and Efficient System ...
https://pubmed.ncbi.nlm.nih.gov/32010678
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning. The cloning technique known as "Nimble Cloning" uses the restriction en …
A cassava common mosaic virus vector for virus-induced gene ...
https://plantmethods.biomedcentral.com › ...
Accordingly, each PCR-generated target fragment with NC adapters could be cloned into the pCsCMV-NC vector via Nimble Cloning. Silencing of ...
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Nimble Cloning试剂盒含Nimble Mix,用于NC克隆反应。 但稀有酶切位点SfiI是NC克隆反应的限制位点。 如果目标基因不含SfiI位点,可直接使用Nimble Mix进行NC克隆反应;如果目标基因含SfiI位点(出现概率较低),则不能用于NC克隆,需要将NC表达载体用SfiI酶切后回收,再和目标基因利用Pre-Nimble Mix进行克隆反应。
An efficient papaya leaf distortion mosaic potyvirus vector for ...
https://www.nature.com › articles
The target fragments for gene silencing can easily be cloned into ... Construction of a stable Nimble Cloning-based PLDMV vector in E. coli ...
Nimble Cloning: A Simple, Versatile, and Efficient System ...
https://europepmc.org/article/PMC/PMC6974442
01.01.2019 · Nimble Cloning enables the direct use of an entry clone for highly efficient cloning, thereby facilitating modular DNA assembly and multi-level construct assembly. In summary, Nimble Cloning is simple, versatile, and efficient, and is a promising system for the standardized molecular cloning required in diverse applications.
Cloning a Datastore - HPE InfoSight
https://infosight.hpe.com › public
The HPE Nimble Storage vCenter plugin can also automate the task of cloning a datastore. With this functionality, you can create one or more clones based on ...
Solved: Nimble Clone to Production Volume - Hewlett ...
https://community.hpe.com/t5/Array-Setup-and-Networking/Nimble-Clone...
11.05.2015 · Re: Nimble Clone to Production Volume Please capture those request Amirul and reach out to the appropriate account team - they can add these use cases to the Request for Engineering to ensure demand for this type of functionality is captured and prioritised
Nimble Cloning: A Simple, Versatile, and Efficient System for ...
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The cloning technique known as “Nimble Cloning” uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the ...
Nimble Cloning(NC克隆) 海南你行生物科技有限公司
ncloning.com
18.07.2021 · Nimble Cloning(NC克隆)为国产标准化分子克隆系统,打破了国外技术在分子克隆领域的长期垄断, 填补了国产分子克隆技术的空白。目前国内已有150多家实验室使用NC克隆,包括10家国家重点实验室。 你行生物的发展目标:1.
(PDF) Nimble Cloning: A Simple, Versatile, and Efficient ...
https://www.researchgate.net/publication/338600025_Nimble_Cloning_A...
15.01.2020 · Nimble Cloning is applicable for the cloning of single or multiple fragments, as well as multi-site cloning. Due also to its simplicity and …