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nimble cloning

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Nimble Cloning试剂盒含Nimble Mix,用于NC克隆反应。 但稀有酶切位点SfiI是NC克隆反应的限制位点。 如果目标基因不含SfiI位点,可直接使用Nimble Mix进行NC克隆反应;如果目标基因含SfiI位点(出现概率较低),则不能用于NC克隆,需要将NC表达载体用SfiI酶切后回收,再和目标基因利用Pre-Nimble Mix进行克隆反应。
Nimble Cloning: A Simple, Versatile, and Efficient System ...
https://pubmed.ncbi.nlm.nih.gov/32010678
Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning. The cloning technique known as "Nimble Cloning" uses the restriction en …
A cassava common mosaic virus vector for virus-induced gene ...
https://plantmethods.biomedcentral.com › ...
Accordingly, each PCR-generated target fragment with NC adapters could be cloned into the pCsCMV-NC vector via Nimble Cloning. Silencing of ...
Cloning a Datastore - HPE InfoSight
https://infosight.hpe.com › public
The HPE Nimble Storage vCenter plugin can also automate the task of cloning a datastore. With this functionality, you can create one or more clones based on ...
Nimble Cloning: A Simple, Versatile, and Efficient System ...
https://europepmc.org/article/PMC/PMC6974442
01.01.2019 · Nimble Cloning enables the direct use of an entry clone for highly efficient cloning, thereby facilitating modular DNA assembly and multi-level construct assembly. In summary, Nimble Cloning is simple, versatile, and efficient, and is a promising system for the standardized molecular cloning required in diverse applications.
Nimble Cloning: A Simple, Versatile, and Efficient ... - Meta
https://www.meta.org › papers › ni...
The cloning technique known as "Nimble Cloning" uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the vector and generate ...
Nimble Cloning: A Simple, Versatile, and Efficient ... - PubMed
https://pubmed.ncbi.nlm.nih.gov › ...
The cloning technique known as "Nimble Cloning" uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the ...
Nimble Cloning: A Simple, Versatile, and Efficient System for ...
https://www.frontiersin.org › full
The cloning technique known as “Nimble Cloning” uses the restriction enzyme, SfiI, in combination with the T5 exonuclease, to linearize the ...
An efficient papaya leaf distortion mosaic potyvirus vector for ...
https://www.nature.com › articles
The target fragments for gene silencing can easily be cloned into ... Construction of a stable Nimble Cloning-based PLDMV vector in E. coli ...
Solved: Nimble Clone to Production Volume - Hewlett ...
https://community.hpe.com/t5/Array-Setup-and-Networking/Nimble-Clone...
11.05.2015 · Re: Nimble Clone to Production Volume Please capture those request Amirul and reach out to the appropriate account team - they can add these use cases to the Request for Engineering to ensure demand for this type of functionality is captured and prioritised
Nimble Cloning(NC克隆) 海南你行生物科技有限公司
ncloning.com
18.07.2021 · Nimble Cloning(NC克隆)为国产标准化分子克隆系统,打破了国外技术在分子克隆领域的长期垄断, 填补了国产分子克隆技术的空白。目前国内已有150多家实验室使用NC克隆,包括10家国家重点实验室。 你行生物的发展目标:1.
Nimble Cloning was applied for a plant expression system. (A)...
https://www.researchgate.net › figure
In this study, we have developed a novel method for standardized molecular cloning. The cloning technique known as “Nimble Cloning” uses the restriction enzyme, ...
(PDF) Nimble Cloning: A Simple, Versatile, and Efficient ...
https://www.researchgate.net/publication/338600025_Nimble_Cloning_A...
15.01.2020 · Nimble Cloning is applicable for the cloning of single or multiple fragments, as well as multi-site cloning. Due also to its simplicity and …